Biomolecular NMR spectroscopy provides invaluable insights into macromolecular structures, dynamics and interactions. Such studies require the production of macromolecules (proteins or nucleic acids) that have been enriched with NMR-visible stable isotopes. Recent advances in isotopic labelling techniques have allowed increasingly complicated biomolecular systems to be investigated by NMR spectroscopy, such as applications to biological machineries with molecular weights exceeding 1 MDa, large ribonucleotide particles and complex membrane protein systems.
Many isotopic labelling schemes and methodologies are available and the choice of the optimum strategy depends greatly on the system being studied and the question being asked. This School will provide both practical and theoretical training in state-of-the-art isotopic-labelling approaches for NMR studies. The participants will gain hands-on experience in a broad range of labelling methods, including innovative protocols for specific labelling, segmental labelling and in vitro expression of RNA and proteins. Participants will be encouraged to bring their own protein and/or RNA constructs for use in practical demonstrations. The School will provide an environment in which attendants will be able to exchange practical experience on protein labelling with instructors and other participants. This lab-based training course will be held at the IBS in Grenoble, France.
The practical school will follow an international workshop on “Advanced Isotopic Labelling Methods for Integrated Structural Biology” organized at IBS from 26th to 29th March 2019. Trainees enrolled in the practical school will also participate in the AILM2019 workshop, allowing them to attend lectures covering the latest developments and applications of isotopic labelling presented by the leaders in the field.